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pE8UCAT [pE8UC, pEU-CAT]
pE8UCAT [pE8UC, pEU-CAT]
規(guī)格:
貨期:
編號:B234816
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pE8UCAT [pE8UC, pEU-CAT]
商品貨號 B234816
Designations pE8UCAT [pE8UC, pEU-CAT]
Depositors G Piaggio
Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: pE8UCAT (plasmid)
Promoters: Promoter none
Construction: pEMBL8-CAT, pUMSSB1
Marker(s):ampR
Construct size (kb): 7.0
Features: insert detection: CAT
marker(s): ampR
promoter: none
replicon: pMB1
terminator: UMS
enhancer: none
Applications
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--5.4, 1.1, 0.4; HindIII--6.6.
The presence of the terminator upstream of the cloning sites of the polylinker prevents transcription that starts at cryptic promoters in the vector sequence.
This is a promoter cloning vector suitable for studying transcriptional activity of very weakly active promoter fragments.
This was constructed by inserting a 1.025 kb SalI fragment from pUMSSB1 containing the strong transcription terminator from the mouse c-mos gene (UMS = upstream mouse sequence) into the EcoRI site of pEMBL8-CAT using EcoRI linkers.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Piaggio G, DeSimone V. A new expression vector to study weak promoters. Focus 12: 83-84, 1990.

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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