| 產(chǎn)品名稱(chēng) |
pCMV-Myc-S1P |
| 商品貨號(hào) |
B237963 |
| Designations |
pCMV-Myc-S1P |
| GenBank Number |
AF078105
|
| Species |
Cricetus cricetus, hamster |
| Depositors |
J Sakai, RB Rawson |
| Applications |
in another host, produces protein sterol-regulated site-1 protease |
| Vector |
Construct size (kb): 10.19999980926514 |
| Insert |
DNA: cDNA Insert Information: Insert size (kb): 4.4 Genome: Hamster Gene symbol: S1P Gene name: sterol-regulated site-1 protease Type of DNA: cDNA Genbank Accession: AF078105 Insert lengths(kb): 4.400000095367432 Gene product: sterol-regulated site-1 protease [S1P] Target Gene: sterol-regulated site-1 protease |
| Insert Size (kb) |
4.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
?Frozen glycerol stock of Escherichia coli HB101 containing the plasmid. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/XbaI -- 5.8, 4.4; EcoRI--9.7, 0.5; HindIII--6.0, 2.4, 1.8. Insert encodes an epitope-tagged version of the protein including aa 1-23, three novel amino acids (GGR), three tandem copies of the 9E10 human c-Myc epitop tag, followed by aa 24 - 1052 of the protein. |
| References |
Sakai J, et al. Molecular identification of sterol-regulated luminal protease that cleaves SREBPs and controls lipid composition of animal cells. Mol. Cell 2: 505-514, 1998. PubMed: 9809072
|
