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prB2
prB2
規(guī)格:
貨期:
編號:B239671
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 prB2
商品貨號 B239671
Designations prB2
Species Homo sapiens, human
Depositors GE Mark
Vector
Construct size (kb): 4.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.600000023841858
Tissue: fetal liver
Gene product: murine leukemia viral (v-raf-1) oncogene homolog 1( Craf1 transforming gene, murine sarcoma 3611 oncogene 1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, Oncogene RAF1) [RAF1]
Alleles: B1, A1, A2, B2
Insert Size (kb) 1.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PvuII--3.0, 1.6; BglII--4.6.
An EcoRI linker was added to the 3' end before cloning into the MCS.
The insert contains NdeI, BglII, HincII, XbaI, ApaI, PvuII, and AccI sites.
Derived from pHB2.
References

Graziano SL, et al. DNA restriction fragment length polymorphisms at either end of the c-raf-1 locus at 3p25. Oncogene Res. 3: 99-103, 1988. PubMed: 2905034

George E Mark, personal communication

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